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STEMCELL Technologies Inc easysep magnetic bead kit
Easysep Magnetic Bead Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+magnetic+beads/easysep+human+t+cell+isolation+kit/bio_rxiv__2025__07__06__663397-127-15-19
Average 90 stars, based on 1 article reviews
easysep magnetic bead kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Purification:

Article Title: Depletion of B cells rejuvenates the peripheral B‐cell compartment but is insufficient to restore immune competence in aging
Article Snippet: .. B-cell purification, stimulations and flow cytometry B cells from mouse spleens or from human peripheral blood were isolated using EasySep magnetic beads (STEMCELL Technologies). ..

Article Title: Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9
Article Snippet: .. CD14 + cells were purified by negative selection using EasySep magnetic beads (STEMCELL Technologies) ( A). ..

Article Title: Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display
Article Snippet: HeLa cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum (FCS) (Thermo Fisher Scientific) at 37°C under 5% CO 2 . .. Monocytes were purified by centrifugation on Ficoll (Sigma-Aldrich) for 20 min at 800 g , and mononucleated cells were subsequently passed through EasySep magnetic beads (STEMCELL Technology). ..

Flow Cytometry:

Article Title: Depletion of B cells rejuvenates the peripheral B‐cell compartment but is insufficient to restore immune competence in aging
Article Snippet: .. B-cell purification, stimulations and flow cytometry B cells from mouse spleens or from human peripheral blood were isolated using EasySep magnetic beads (STEMCELL Technologies). ..

Isolation:

Article Title: Depletion of B cells rejuvenates the peripheral B‐cell compartment but is insufficient to restore immune competence in aging
Article Snippet: .. B-cell purification, stimulations and flow cytometry B cells from mouse spleens or from human peripheral blood were isolated using EasySep magnetic beads (STEMCELL Technologies). ..

Article Title: Use of CDK inhibitors to enhance growth and self-renewal of progenitor cells
Article Snippet: FACS data was analyzed using the FlowJo software (Tree Star Inc., CA). .. In some experiments, S0 cells were isolated from fetal liver cells using EasySep magnetic beads (StemCell Technologies) by negative sorting for CD71, Ter119, Gr1, Mac1 and CD41. .. Antibodies Used in Flow Cytometric and EasySep Purifications: PE/Cy7 rat anti-mouse CD71 (RI7217) (BioLegend 113812) PE rat anti-mouse Ter119 (Ter119) (BD Biosciences 553673) APC rat anti-mouse Ter119 (Ter119) (BD Biosciences 557909) biotin rat anti-mouse CD71 (C2) (BD Biosciences 557416) biotin rat anti-mouse Ter119 (BD Biosciences 553672) biotin rat anti-mouse Ly-6G and Ly-6C/Gr1 (RB6-8C5) (BD Biosciences 553125) biotin rat anti-mouse CD11b/Mac1 (M1/70) (BD Biosciences 557395) biotin rat anti-mouse CD41 (MWReg30) (Thermo Scientific MA1-82655) FITC Rat Anti-Mouse Ly-6G and Ly-6C/Gr1 (RB6-8C5) (BD Biosciences 553127) FITC rat anti-mouse CD11b/Mac1 (M1/70) (BD Biosciences 557396) FITC rat anti-mouse CD41 (MWReg30) (BD Biosciences 553848) FITC rat anti-mouse CD45R/B220 (RA3-6B2) (BD Biosciences 553087) FITC hamster anti-mouse CD3e (145-2C11) (BD Biosciences 553061) PE Annexin V (BD Biosciences 556421) Alexa Fluor 488 mouse anti-H2AX (pS139) (N1-431) (BD Biosciences 560445) Isolation of G1 and S Phase Cells from S0 and S1 Erythroid Subsets E13.5 fetal livers were harvested from wild type BALB/cJ mice (Jackson laboratory stock #000651), were mechanically dissociated, resuspended at 106 cells/ml, and maintained at 37° C. for 15 min with IMDM (L-glutamine, 25 mM HEPES) (Gibco), 20% fetal calf serum (Gibco, HyClone), 100 U/ml penicillin/streptomycin (Invitrogen), 10−4M β-mercapthoethanol (Sigma) and 2 U/ml Epo.

Article Title: Use of CDK inhibitors to enhance growth and self-renewal of progenitor cells
Article Snippet: To test the effect of roscovitine on p57KIP2−/− fetal liver cells in expansion culture, cells were grown and maintained as described above with the additional supplemented daily of 0.5 μM roscovitine (EMD Millipore 557360). .. To switch to differentiation medium, after being maintained in expansion medium for 4 to 5 days, Ter119-negative cells were isolated again using EasySep magnetic beads (StemCell Technologies). .. Cells were then transferred to IMDM (L-glutamine, 25 mM HEPES) (Gibco), 20% fetal calf serum (Gibco, HyClone), 100 U/ml penicillin/streptomycin (Invitrogen), 104 M β-mercapthoethanol (Sigma) and 2 U/ml Epo.

Article Title: Global increase in replication fork speed during a p57 KIP2 -regulated erythroid cell fate switch
Article Snippet: To test the effect of roscovitine on p57 KIP2−/− fetal liver cells in expansion culture, cells were grown and maintained as described above with the additional daily supplement of 0.5 μM roscovitine (EMD Millipore 557360). .. To switch to differentiation medium, after being maintained in expansion medium for 4 to 5 days, Ter119-negative cells were isolated again using EasySep magnetic beads (StemCell Technologies). .. Cells were then transferred to IMDM ( l -glutamine, 25 mM Hepes) (Gibco), 20% fetal calf serum (Gibco, HyClone), penicillin/streptomycin (100 U/ml; Invitrogen), 10 −4 M β-mercaptoethanol (Sigma), and Epo (2 U/ml).

Article Title: Methods for treating systemic lupus erythematosus with an anti-apolipoprotein B antibody
Article Snippet: Color was developed with DAB Detection Kit (Vector Labs, CA, USA) binding to the biotinylated antibody and immune stained areas were quantified in BioPix i.Q 2.0 software. .. In vitro Induction of Tregs Through Antigen Presentation CD11c+ dendritic cells were isolated from a MRL/lpr ApoE−/− spleen using CD11c positive selection kit and EasySep magnetic beads (StemCell Technologies). .. CD4+ T cells were isolated using CD4 negative selection kit (StemCell Technologies) followed by isolation of CD25−CD4+ cells were isolated using CD25 positive selection kit (StemCell Technologies).

Magnetic Beads:

Article Title: Depletion of B cells rejuvenates the peripheral B‐cell compartment but is insufficient to restore immune competence in aging
Article Snippet: .. B-cell purification, stimulations and flow cytometry B cells from mouse spleens or from human peripheral blood were isolated using EasySep magnetic beads (STEMCELL Technologies). ..

Article Title: Use of CDK inhibitors to enhance growth and self-renewal of progenitor cells
Article Snippet: FACS data was analyzed using the FlowJo software (Tree Star Inc., CA). .. In some experiments, S0 cells were isolated from fetal liver cells using EasySep magnetic beads (StemCell Technologies) by negative sorting for CD71, Ter119, Gr1, Mac1 and CD41. .. Antibodies Used in Flow Cytometric and EasySep Purifications: PE/Cy7 rat anti-mouse CD71 (RI7217) (BioLegend 113812) PE rat anti-mouse Ter119 (Ter119) (BD Biosciences 553673) APC rat anti-mouse Ter119 (Ter119) (BD Biosciences 557909) biotin rat anti-mouse CD71 (C2) (BD Biosciences 557416) biotin rat anti-mouse Ter119 (BD Biosciences 553672) biotin rat anti-mouse Ly-6G and Ly-6C/Gr1 (RB6-8C5) (BD Biosciences 553125) biotin rat anti-mouse CD11b/Mac1 (M1/70) (BD Biosciences 557395) biotin rat anti-mouse CD41 (MWReg30) (Thermo Scientific MA1-82655) FITC Rat Anti-Mouse Ly-6G and Ly-6C/Gr1 (RB6-8C5) (BD Biosciences 553127) FITC rat anti-mouse CD11b/Mac1 (M1/70) (BD Biosciences 557396) FITC rat anti-mouse CD41 (MWReg30) (BD Biosciences 553848) FITC rat anti-mouse CD45R/B220 (RA3-6B2) (BD Biosciences 553087) FITC hamster anti-mouse CD3e (145-2C11) (BD Biosciences 553061) PE Annexin V (BD Biosciences 556421) Alexa Fluor 488 mouse anti-H2AX (pS139) (N1-431) (BD Biosciences 560445) Isolation of G1 and S Phase Cells from S0 and S1 Erythroid Subsets E13.5 fetal livers were harvested from wild type BALB/cJ mice (Jackson laboratory stock #000651), were mechanically dissociated, resuspended at 106 cells/ml, and maintained at 37° C. for 15 min with IMDM (L-glutamine, 25 mM HEPES) (Gibco), 20% fetal calf serum (Gibco, HyClone), 100 U/ml penicillin/streptomycin (Invitrogen), 10−4M β-mercapthoethanol (Sigma) and 2 U/ml Epo.

Article Title: HOXA9 has the hallmarks of a biological switch with implications in blood cancers
Article Snippet: .. Red blood cell lysis was achieved by treatment with Ammonium Chloride solution (StemCell Technologies) and lineage depletion was performed by immunomagnetic selection of mature lineage cells using the haematopoietic stem progenitor cell (HSPC) enrichment cocktail and EasySep magnetic beads (StemCell technologies). ..

Article Title: Use of CDK inhibitors to enhance growth and self-renewal of progenitor cells
Article Snippet: To test the effect of roscovitine on p57KIP2−/− fetal liver cells in expansion culture, cells were grown and maintained as described above with the additional supplemented daily of 0.5 μM roscovitine (EMD Millipore 557360). .. To switch to differentiation medium, after being maintained in expansion medium for 4 to 5 days, Ter119-negative cells were isolated again using EasySep magnetic beads (StemCell Technologies). .. Cells were then transferred to IMDM (L-glutamine, 25 mM HEPES) (Gibco), 20% fetal calf serum (Gibco, HyClone), 100 U/ml penicillin/streptomycin (Invitrogen), 104 M β-mercapthoethanol (Sigma) and 2 U/ml Epo.

Article Title: Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9
Article Snippet: .. CD14 + cells were purified by negative selection using EasySep magnetic beads (STEMCELL Technologies) ( A). ..

Article Title: Global increase in replication fork speed during a p57 KIP2 -regulated erythroid cell fate switch
Article Snippet: To test the effect of roscovitine on p57 KIP2−/− fetal liver cells in expansion culture, cells were grown and maintained as described above with the additional daily supplement of 0.5 μM roscovitine (EMD Millipore 557360). .. To switch to differentiation medium, after being maintained in expansion medium for 4 to 5 days, Ter119-negative cells were isolated again using EasySep magnetic beads (StemCell Technologies). .. Cells were then transferred to IMDM ( l -glutamine, 25 mM Hepes) (Gibco), 20% fetal calf serum (Gibco, HyClone), penicillin/streptomycin (100 U/ml; Invitrogen), 10 −4 M β-mercaptoethanol (Sigma), and Epo (2 U/ml).

Article Title: Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display
Article Snippet: HeLa cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum (FCS) (Thermo Fisher Scientific) at 37°C under 5% CO 2 . .. Monocytes were purified by centrifugation on Ficoll (Sigma-Aldrich) for 20 min at 800 g , and mononucleated cells were subsequently passed through EasySep magnetic beads (STEMCELL Technology). ..

Article Title: Methods for treating systemic lupus erythematosus with an anti-apolipoprotein B antibody
Article Snippet: Color was developed with DAB Detection Kit (Vector Labs, CA, USA) binding to the biotinylated antibody and immune stained areas were quantified in BioPix i.Q 2.0 software. .. In vitro Induction of Tregs Through Antigen Presentation CD11c+ dendritic cells were isolated from a MRL/lpr ApoE−/− spleen using CD11c positive selection kit and EasySep magnetic beads (StemCell Technologies). .. CD4+ T cells were isolated using CD4 negative selection kit (StemCell Technologies) followed by isolation of CD25−CD4+ cells were isolated using CD25 positive selection kit (StemCell Technologies).

Red Blood Cell Lysis:

Article Title: HOXA9 has the hallmarks of a biological switch with implications in blood cancers
Article Snippet: .. Red blood cell lysis was achieved by treatment with Ammonium Chloride solution (StemCell Technologies) and lineage depletion was performed by immunomagnetic selection of mature lineage cells using the haematopoietic stem progenitor cell (HSPC) enrichment cocktail and EasySep magnetic beads (StemCell technologies). ..

Selection:

Article Title: HOXA9 has the hallmarks of a biological switch with implications in blood cancers
Article Snippet: .. Red blood cell lysis was achieved by treatment with Ammonium Chloride solution (StemCell Technologies) and lineage depletion was performed by immunomagnetic selection of mature lineage cells using the haematopoietic stem progenitor cell (HSPC) enrichment cocktail and EasySep magnetic beads (StemCell technologies). ..

Article Title: Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9
Article Snippet: .. CD14 + cells were purified by negative selection using EasySep magnetic beads (STEMCELL Technologies) ( A). ..

Article Title: Methods for treating systemic lupus erythematosus with an anti-apolipoprotein B antibody
Article Snippet: Color was developed with DAB Detection Kit (Vector Labs, CA, USA) binding to the biotinylated antibody and immune stained areas were quantified in BioPix i.Q 2.0 software. .. In vitro Induction of Tregs Through Antigen Presentation CD11c+ dendritic cells were isolated from a MRL/lpr ApoE−/− spleen using CD11c positive selection kit and EasySep magnetic beads (StemCell Technologies). .. CD4+ T cells were isolated using CD4 negative selection kit (StemCell Technologies) followed by isolation of CD25−CD4+ cells were isolated using CD25 positive selection kit (StemCell Technologies).

Centrifugation:

Article Title: Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display
Article Snippet: HeLa cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum (FCS) (Thermo Fisher Scientific) at 37°C under 5% CO 2 . .. Monocytes were purified by centrifugation on Ficoll (Sigma-Aldrich) for 20 min at 800 g , and mononucleated cells were subsequently passed through EasySep magnetic beads (STEMCELL Technology). ..

In Vitro:

Article Title: Methods for treating systemic lupus erythematosus with an anti-apolipoprotein B antibody
Article Snippet: Color was developed with DAB Detection Kit (Vector Labs, CA, USA) binding to the biotinylated antibody and immune stained areas were quantified in BioPix i.Q 2.0 software. .. In vitro Induction of Tregs Through Antigen Presentation CD11c+ dendritic cells were isolated from a MRL/lpr ApoE−/− spleen using CD11c positive selection kit and EasySep magnetic beads (StemCell Technologies). .. CD4+ T cells were isolated using CD4 negative selection kit (StemCell Technologies) followed by isolation of CD25−CD4+ cells were isolated using CD25 positive selection kit (StemCell Technologies).

Immunopeptidomics:

Article Title: Methods for treating systemic lupus erythematosus with an anti-apolipoprotein B antibody
Article Snippet: Color was developed with DAB Detection Kit (Vector Labs, CA, USA) binding to the biotinylated antibody and immune stained areas were quantified in BioPix i.Q 2.0 software. .. In vitro Induction of Tregs Through Antigen Presentation CD11c+ dendritic cells were isolated from a MRL/lpr ApoE−/− spleen using CD11c positive selection kit and EasySep magnetic beads (StemCell Technologies). .. CD4+ T cells were isolated using CD4 negative selection kit (StemCell Technologies) followed by isolation of CD25−CD4+ cells were isolated using CD25 positive selection kit (StemCell Technologies).



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Image Search Results


( A ) Start and end time distribution of CD4 + T-cell tracks on non-stimulated or TNF- or IL-1β-stimulated primary mouse brain microvascular endothelial cells (pMBMECs). Under-flow migration tracker ( UFM Track) correctly captures increased T-cell detachment from the non-stimulated pMBMECs after 5 min when the flow is increased to physiological levels. ( B ) Quantification of CD4 + T-cell behavior in the respective categories obtained on non-stimulated and TNF- and IL-1β-stimulated pMBMECs. Error bars show the statistical error of the mean (see text for details). ( C–H ) Motility parameters of the crawling CD4 + T cells were obtained for the three endothelial stimulatory conditions. Distributions of T-cell path length ( C ), displacement ( D ), crawling speed (path/time) ( E ), migration speed (displacement/time) ( F ), variability of instantaneous T-cell crawling speed along the track (standard deviation, G ), and meandering index ( H ). ( I ) Distribution of CD4 + T-cell accelerated movement (AM) speed is a proxy metric for the T-cell adhesion to the healthy or inflamed endothelium. ( J ) Migration speed distribution of the transmigrated CD4 + T cells. Stimulation applied to the luminal side of pMBMECs affects T-cell migration at the abluminal side of pMBMECs after their transmigration. FT – T cells performed full transmigration; UT – T cells performed uncompleted transmigration. Statistical tests are performed with the Mann-Whitney U test. Statistical significance is indicated as follows: ns – Not significant (p > 0.05); * – p ≤ 0.05; ** – p ≤ 0.01; *** – p ≤ 0.001; **** – p ≤ 0.0001.

Journal: eLife

Article Title: UFMTrack, an Under-Flow Migration Tracker enabling analysis of the entire multi-step immune cell extravasation cascade across the blood-brain barrier in microfluidic devices

doi: 10.7554/eLife.91150

Figure Lengend Snippet: ( A ) Start and end time distribution of CD4 + T-cell tracks on non-stimulated or TNF- or IL-1β-stimulated primary mouse brain microvascular endothelial cells (pMBMECs). Under-flow migration tracker ( UFM Track) correctly captures increased T-cell detachment from the non-stimulated pMBMECs after 5 min when the flow is increased to physiological levels. ( B ) Quantification of CD4 + T-cell behavior in the respective categories obtained on non-stimulated and TNF- and IL-1β-stimulated pMBMECs. Error bars show the statistical error of the mean (see text for details). ( C–H ) Motility parameters of the crawling CD4 + T cells were obtained for the three endothelial stimulatory conditions. Distributions of T-cell path length ( C ), displacement ( D ), crawling speed (path/time) ( E ), migration speed (displacement/time) ( F ), variability of instantaneous T-cell crawling speed along the track (standard deviation, G ), and meandering index ( H ). ( I ) Distribution of CD4 + T-cell accelerated movement (AM) speed is a proxy metric for the T-cell adhesion to the healthy or inflamed endothelium. ( J ) Migration speed distribution of the transmigrated CD4 + T cells. Stimulation applied to the luminal side of pMBMECs affects T-cell migration at the abluminal side of pMBMECs after their transmigration. FT – T cells performed full transmigration; UT – T cells performed uncompleted transmigration. Statistical tests are performed with the Mann-Whitney U test. Statistical significance is indicated as follows: ns – Not significant (p > 0.05); * – p ≤ 0.05; ** – p ≤ 0.01; *** – p ≤ 0.001; **** – p ≤ 0.0001.

Article Snippet: 2D2 and OT-I cells were isolated respectively with magnetic CD4 + and CD8 + T-cell selection beads (EasySep, STEMCELL Technologies).

Techniques: Migration, Standard Deviation, Transmigration Assay, MANN-WHITNEY

Under-flow migration tracker ( UFM Track) is able to successfully reconstruct tracks of migrating cells of different sizes. CD4 and CD8 – mouse CD4 + and CD8 + cells, BMDM – bone marrow-derived macrophages, hT – human T cells, hPBMC – human peripheral blood mononuclear cells. The statistical tests are performed with the Mann-Whitney U test. Statistical significance is indicated as follows: ns – Not significant (p > 0.05); * – p ≤ 0.05; ** – p ≤ 0.01; *** – p ≤ 0.001; **** – p ≤ 0.0001.

Journal: eLife

Article Title: UFMTrack, an Under-Flow Migration Tracker enabling analysis of the entire multi-step immune cell extravasation cascade across the blood-brain barrier in microfluidic devices

doi: 10.7554/eLife.91150

Figure Lengend Snippet: Under-flow migration tracker ( UFM Track) is able to successfully reconstruct tracks of migrating cells of different sizes. CD4 and CD8 – mouse CD4 + and CD8 + cells, BMDM – bone marrow-derived macrophages, hT – human T cells, hPBMC – human peripheral blood mononuclear cells. The statistical tests are performed with the Mann-Whitney U test. Statistical significance is indicated as follows: ns – Not significant (p > 0.05); * – p ≤ 0.05; ** – p ≤ 0.01; *** – p ≤ 0.001; **** – p ≤ 0.0001.

Article Snippet: 2D2 and OT-I cells were isolated respectively with magnetic CD4 + and CD8 + T-cell selection beads (EasySep, STEMCELL Technologies).

Techniques: Migration, Derivative Assay, MANN-WHITNEY